Journal: Folia biologica

Article Title: Up-regulation of MiR-146b-5p Inhibits Fibrotic Lung Pericytes via Inactivation of the Notch1/PDGFRβ/ROCK1 Pathway.

doi: 10.14712/fb2022068050180

Figure Lengend Snippet: Fig. 3. MiR-146b-5p up-regulation inhibited proliferation of pericytes in mice with lung fibrosis. (A) Pericytes were identified by CD13 and PDGFRβ staining. (B) Pericytes were transfected with NC mimics or miR-146b-5p mimics. The level of miR-146b-5p in the pericytes was assessed by RT-qPCR. (C) The viability of pericytes was assessed by CCK8 assay. (D) The cell cycle distribution was assessed by flow cytometry. (E) The expression of α-SMA and NG2 in the pericytes was evaluated by immunofluorescence staining. *P < 0.05.

Article Snippet: To isolate pericytes, cells were incubated with anti-PDGFRβ polyclonal antibody (#3169, Cell Signaling Technology, Danvers, MA).

Techniques: Staining, Transfection, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Expressing, Immunofluorescence

Journal: Folia biologica

Article Title: Up-regulation of MiR-146b-5p Inhibits Fibrotic Lung Pericytes via Inactivation of the Notch1/PDGFRβ/ROCK1 Pathway.

doi: 10.14712/fb2022068050180

Figure Lengend Snippet: Fig. 4. Up-regulation of miR-146b-5p inhibited the fibrosis in pericytes through inactivation of the Notch1/PDGFRβ/ ROCK1 pathway. (A) The mRNA levels of Notch1, PDGFRβ and ROCK1 in the pericytes of mice with lung fibrosis were investigated by RT-qPCR. (B) The protein levels of Notch1, PDGFRβ and ROCK1 in the pericytes of mice with lung fi- brosis were assessed by Western blot. *P < 0.05.

Article Snippet: To isolate pericytes, cells were incubated with anti-PDGFRβ polyclonal antibody (#3169, Cell Signaling Technology, Danvers, MA).

Techniques: Quantitative RT-PCR, Western Blot